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mouse α isl1 antibodies  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse α isl1 antibodies
    Glucose levels modulate β-cell enriched mRNAs, including <t>Isl1</t> . A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 respectively) from primary mouse islets after overnight culturing in media with increasing glucose concentrations (4 mM, 7.8 mM, 15.6 mM, and 31 mM). Low glucose (4 mM) was set as onefold, and 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I. Top: Western blotting of Ins-1 cell extracts for LDB1, ISL1, or MAFA after 4 hr or overnight treatment with similar glucose concentrations as above. Actin was included as a loading control. Bottom: Densitometry quantification of 4 h or overnight treated LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA.
    Mouse α Isl1 Antibodies, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 1052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse α isl1 antibodies/product/Developmental Studies Hybridoma Bank
    Average 97 stars, based on 1052 article reviews
    mouse α isl1 antibodies - by Bioz Stars, 2026-03
    97/100 stars

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    1) Product Images from "LDB1-mediated transcriptional complexes are sensitive to islet stress"

    Article Title: LDB1-mediated transcriptional complexes are sensitive to islet stress

    Journal: Islets

    doi: 10.1080/19382014.2021.2016028

    Glucose levels modulate β-cell enriched mRNAs, including Isl1 . A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 respectively) from primary mouse islets after overnight culturing in media with increasing glucose concentrations (4 mM, 7.8 mM, 15.6 mM, and 31 mM). Low glucose (4 mM) was set as onefold, and 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I. Top: Western blotting of Ins-1 cell extracts for LDB1, ISL1, or MAFA after 4 hr or overnight treatment with similar glucose concentrations as above. Actin was included as a loading control. Bottom: Densitometry quantification of 4 h or overnight treated LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA.
    Figure Legend Snippet: Glucose levels modulate β-cell enriched mRNAs, including Isl1 . A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 respectively) from primary mouse islets after overnight culturing in media with increasing glucose concentrations (4 mM, 7.8 mM, 15.6 mM, and 31 mM). Low glucose (4 mM) was set as onefold, and 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I. Top: Western blotting of Ins-1 cell extracts for LDB1, ISL1, or MAFA after 4 hr or overnight treatment with similar glucose concentrations as above. Actin was included as a loading control. Bottom: Densitometry quantification of 4 h or overnight treated LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA.

    Techniques Used: Western Blot, Control

    Palmitate treatment downregulates LDB1 and SSBP3 co-regulator levels. A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 , respectively) from primary mouse islets after overnight culturing in media with control BSA (set as 1-fold) or palmitic acid (PA, 0.5 mM). 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I . Left: Western blot of LDB1, MAFA, and ISL1 in Ins-1 cell extracts after overnight treatment with BSA control or 0.5 mM PA. Actin was included as loading control. Right: Densitometry quantification of LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; ***, P < .001; ****, P < .0001, based on Student’s t-test.
    Figure Legend Snippet: Palmitate treatment downregulates LDB1 and SSBP3 co-regulator levels. A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 , respectively) from primary mouse islets after overnight culturing in media with control BSA (set as 1-fold) or palmitic acid (PA, 0.5 mM). 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I . Left: Western blot of LDB1, MAFA, and ISL1 in Ins-1 cell extracts after overnight treatment with BSA control or 0.5 mM PA. Actin was included as loading control. Right: Densitometry quantification of LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; ***, P < .001; ****, P < .0001, based on Student’s t-test.

    Techniques Used: Control, Western Blot

    Critical β-cell factors are reduced upon cytokine treatment in mouse and human islets. A-F. Relative mRNA quantification of various β-cell transcriptional regulator genes ( MafA, Pdx1, Nkx6.1, Ldb1, Isl1 , and SSBP3 , respectively) from primary mouse islets after 4 h culturing with single cytokines (Tnfα, Ifnγ, IL-1β) or a cocktail of each, as compared to PBS vehicle controls (set to 1-fold). 36B4 was used as the housekeeping gene; n = 3–4 for each treatment group. G. Left: LDB1, MAFA, NKX6.1, and ISL1 Western blot with Ins-1 cell extracts after 4 h or overnight treatment with single cytokines, or a cocktail, as compared to control. Actin was included as loading control. Right: Densitometry quantification of 4 h or overnight treated LDB1, MAFA, NKX6.1, or ISL1 Western blot protein levels, normalized to Actin. H. Human islets were treated with a DMSO vehicle or a cytokine cocktail, , then mRNA measured. 18S was used as the housekeeping gene; n = 3. I. Quantification of β-cell mRNAs from primary mouse islets after siRNA-mediated Ldb1 knockdown using Gapdh as the housekeeping gene; n = 3. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA or Student’s t-test.
    Figure Legend Snippet: Critical β-cell factors are reduced upon cytokine treatment in mouse and human islets. A-F. Relative mRNA quantification of various β-cell transcriptional regulator genes ( MafA, Pdx1, Nkx6.1, Ldb1, Isl1 , and SSBP3 , respectively) from primary mouse islets after 4 h culturing with single cytokines (Tnfα, Ifnγ, IL-1β) or a cocktail of each, as compared to PBS vehicle controls (set to 1-fold). 36B4 was used as the housekeeping gene; n = 3–4 for each treatment group. G. Left: LDB1, MAFA, NKX6.1, and ISL1 Western blot with Ins-1 cell extracts after 4 h or overnight treatment with single cytokines, or a cocktail, as compared to control. Actin was included as loading control. Right: Densitometry quantification of 4 h or overnight treated LDB1, MAFA, NKX6.1, or ISL1 Western blot protein levels, normalized to Actin. H. Human islets were treated with a DMSO vehicle or a cytokine cocktail, , then mRNA measured. 18S was used as the housekeeping gene; n = 3. I. Quantification of β-cell mRNAs from primary mouse islets after siRNA-mediated Ldb1 knockdown using Gapdh as the housekeeping gene; n = 3. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA or Student’s t-test.

    Techniques Used: Western Blot, Control, Knockdown

    Glucose and cytokines regulate LDB1:ISL1 protein interactions in Min6 β-cells. A-C. PLA immunofluorescence (red focal spots) and DAPI nuclear signals (blue) in Min6 cells treated with (A) control low glucose, (B) high-glucose, and (C) TNFα/IL-1β/IFNγ cytokine cocktail. Insets are 200% magnified images to show greater detail, which are taken from the main field, denoted by dashed white boxes. D. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (red focal spots) in Min6 cells in A-C. E-F. PLA immunofluorescence (red) and DAPI nuclear signals (blue) in Min6 cells treated with (E.) Palmitate control-BSA or (F.) palmitic acid (PA). G. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (focal spots) in Min6 cells in E-F. n = 3; scale bar = 20 μm. ****, P < .0001 based on Student’s t-test.
    Figure Legend Snippet: Glucose and cytokines regulate LDB1:ISL1 protein interactions in Min6 β-cells. A-C. PLA immunofluorescence (red focal spots) and DAPI nuclear signals (blue) in Min6 cells treated with (A) control low glucose, (B) high-glucose, and (C) TNFα/IL-1β/IFNγ cytokine cocktail. Insets are 200% magnified images to show greater detail, which are taken from the main field, denoted by dashed white boxes. D. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (red focal spots) in Min6 cells in A-C. E-F. PLA immunofluorescence (red) and DAPI nuclear signals (blue) in Min6 cells treated with (E.) Palmitate control-BSA or (F.) palmitic acid (PA). G. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (focal spots) in Min6 cells in E-F. n = 3; scale bar = 20 μm. ****, P < .0001 based on Student’s t-test.

    Techniques Used: Immunofluorescence, Control



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    mouse α isl1 antibodies  (Developmental Studies Hybridoma Bank)
    97
    Developmental Studies Hybridoma Bank mouse α isl1 antibodies
    Glucose levels modulate β-cell enriched mRNAs, including <t>Isl1</t> . A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 respectively) from primary mouse islets after overnight culturing in media with increasing glucose concentrations (4 mM, 7.8 mM, 15.6 mM, and 31 mM). Low glucose (4 mM) was set as onefold, and 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I. Top: Western blotting of Ins-1 cell extracts for LDB1, ISL1, or MAFA after 4 hr or overnight treatment with similar glucose concentrations as above. Actin was included as a loading control. Bottom: Densitometry quantification of 4 h or overnight treated LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA.
    Mouse α Isl1 Antibodies, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse α isl1 antibodies/product/Developmental Studies Hybridoma Bank
    Average 97 stars, based on 1 article reviews
    mouse α isl1 antibodies - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Glucose levels modulate β-cell enriched mRNAs, including Isl1 . A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 respectively) from primary mouse islets after overnight culturing in media with increasing glucose concentrations (4 mM, 7.8 mM, 15.6 mM, and 31 mM). Low glucose (4 mM) was set as onefold, and 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I. Top: Western blotting of Ins-1 cell extracts for LDB1, ISL1, or MAFA after 4 hr or overnight treatment with similar glucose concentrations as above. Actin was included as a loading control. Bottom: Densitometry quantification of 4 h or overnight treated LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA.

    Journal: Islets

    Article Title: LDB1-mediated transcriptional complexes are sensitive to islet stress

    doi: 10.1080/19382014.2021.2016028

    Figure Lengend Snippet: Glucose levels modulate β-cell enriched mRNAs, including Isl1 . A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 respectively) from primary mouse islets after overnight culturing in media with increasing glucose concentrations (4 mM, 7.8 mM, 15.6 mM, and 31 mM). Low glucose (4 mM) was set as onefold, and 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I. Top: Western blotting of Ins-1 cell extracts for LDB1, ISL1, or MAFA after 4 hr or overnight treatment with similar glucose concentrations as above. Actin was included as a loading control. Bottom: Densitometry quantification of 4 h or overnight treated LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA.

    Article Snippet: After stimuli treatment for 4 hr in low (2.5 mM) or high (25 mM) glucose, the cells were formaldehyde fixed and then incubated with rabbit α-LDB1 (kind gift from Dr. Paul Love, NIH) and mouse α-ISL1 antibodies (39.4D5-c, Developmental Studies Hybridoma Bank) prior to PLA procedure.

    Techniques: Western Blot, Control

    Palmitate treatment downregulates LDB1 and SSBP3 co-regulator levels. A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 , respectively) from primary mouse islets after overnight culturing in media with control BSA (set as 1-fold) or palmitic acid (PA, 0.5 mM). 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I . Left: Western blot of LDB1, MAFA, and ISL1 in Ins-1 cell extracts after overnight treatment with BSA control or 0.5 mM PA. Actin was included as loading control. Right: Densitometry quantification of LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; ***, P < .001; ****, P < .0001, based on Student’s t-test.

    Journal: Islets

    Article Title: LDB1-mediated transcriptional complexes are sensitive to islet stress

    doi: 10.1080/19382014.2021.2016028

    Figure Lengend Snippet: Palmitate treatment downregulates LDB1 and SSBP3 co-regulator levels. A-H. Relative mRNA quantification of various β-cell factors ( Insulin I, Insulin II, MafA, Pdx1, Nkx6.1, SSBP3, Isl1 , and Ldb1 , respectively) from primary mouse islets after overnight culturing in media with control BSA (set as 1-fold) or palmitic acid (PA, 0.5 mM). 36B4 was used as the housekeeping gene; n = 3 for each treatment group. I . Left: Western blot of LDB1, MAFA, and ISL1 in Ins-1 cell extracts after overnight treatment with BSA control or 0.5 mM PA. Actin was included as loading control. Right: Densitometry quantification of LDB1, ISL1, or MAFA Western blot protein levels, normalized to Actin. *, P < .05; ***, P < .001; ****, P < .0001, based on Student’s t-test.

    Article Snippet: After stimuli treatment for 4 hr in low (2.5 mM) or high (25 mM) glucose, the cells were formaldehyde fixed and then incubated with rabbit α-LDB1 (kind gift from Dr. Paul Love, NIH) and mouse α-ISL1 antibodies (39.4D5-c, Developmental Studies Hybridoma Bank) prior to PLA procedure.

    Techniques: Control, Western Blot

    Critical β-cell factors are reduced upon cytokine treatment in mouse and human islets. A-F. Relative mRNA quantification of various β-cell transcriptional regulator genes ( MafA, Pdx1, Nkx6.1, Ldb1, Isl1 , and SSBP3 , respectively) from primary mouse islets after 4 h culturing with single cytokines (Tnfα, Ifnγ, IL-1β) or a cocktail of each, as compared to PBS vehicle controls (set to 1-fold). 36B4 was used as the housekeeping gene; n = 3–4 for each treatment group. G. Left: LDB1, MAFA, NKX6.1, and ISL1 Western blot with Ins-1 cell extracts after 4 h or overnight treatment with single cytokines, or a cocktail, as compared to control. Actin was included as loading control. Right: Densitometry quantification of 4 h or overnight treated LDB1, MAFA, NKX6.1, or ISL1 Western blot protein levels, normalized to Actin. H. Human islets were treated with a DMSO vehicle or a cytokine cocktail, , then mRNA measured. 18S was used as the housekeeping gene; n = 3. I. Quantification of β-cell mRNAs from primary mouse islets after siRNA-mediated Ldb1 knockdown using Gapdh as the housekeeping gene; n = 3. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA or Student’s t-test.

    Journal: Islets

    Article Title: LDB1-mediated transcriptional complexes are sensitive to islet stress

    doi: 10.1080/19382014.2021.2016028

    Figure Lengend Snippet: Critical β-cell factors are reduced upon cytokine treatment in mouse and human islets. A-F. Relative mRNA quantification of various β-cell transcriptional regulator genes ( MafA, Pdx1, Nkx6.1, Ldb1, Isl1 , and SSBP3 , respectively) from primary mouse islets after 4 h culturing with single cytokines (Tnfα, Ifnγ, IL-1β) or a cocktail of each, as compared to PBS vehicle controls (set to 1-fold). 36B4 was used as the housekeeping gene; n = 3–4 for each treatment group. G. Left: LDB1, MAFA, NKX6.1, and ISL1 Western blot with Ins-1 cell extracts after 4 h or overnight treatment with single cytokines, or a cocktail, as compared to control. Actin was included as loading control. Right: Densitometry quantification of 4 h or overnight treated LDB1, MAFA, NKX6.1, or ISL1 Western blot protein levels, normalized to Actin. H. Human islets were treated with a DMSO vehicle or a cytokine cocktail, , then mRNA measured. 18S was used as the housekeeping gene; n = 3. I. Quantification of β-cell mRNAs from primary mouse islets after siRNA-mediated Ldb1 knockdown using Gapdh as the housekeeping gene; n = 3. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001 based on one-way ANOVA or Student’s t-test.

    Article Snippet: After stimuli treatment for 4 hr in low (2.5 mM) or high (25 mM) glucose, the cells were formaldehyde fixed and then incubated with rabbit α-LDB1 (kind gift from Dr. Paul Love, NIH) and mouse α-ISL1 antibodies (39.4D5-c, Developmental Studies Hybridoma Bank) prior to PLA procedure.

    Techniques: Western Blot, Control, Knockdown

    Glucose and cytokines regulate LDB1:ISL1 protein interactions in Min6 β-cells. A-C. PLA immunofluorescence (red focal spots) and DAPI nuclear signals (blue) in Min6 cells treated with (A) control low glucose, (B) high-glucose, and (C) TNFα/IL-1β/IFNγ cytokine cocktail. Insets are 200% magnified images to show greater detail, which are taken from the main field, denoted by dashed white boxes. D. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (red focal spots) in Min6 cells in A-C. E-F. PLA immunofluorescence (red) and DAPI nuclear signals (blue) in Min6 cells treated with (E.) Palmitate control-BSA or (F.) palmitic acid (PA). G. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (focal spots) in Min6 cells in E-F. n = 3; scale bar = 20 μm. ****, P < .0001 based on Student’s t-test.

    Journal: Islets

    Article Title: LDB1-mediated transcriptional complexes are sensitive to islet stress

    doi: 10.1080/19382014.2021.2016028

    Figure Lengend Snippet: Glucose and cytokines regulate LDB1:ISL1 protein interactions in Min6 β-cells. A-C. PLA immunofluorescence (red focal spots) and DAPI nuclear signals (blue) in Min6 cells treated with (A) control low glucose, (B) high-glucose, and (C) TNFα/IL-1β/IFNγ cytokine cocktail. Insets are 200% magnified images to show greater detail, which are taken from the main field, denoted by dashed white boxes. D. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (red focal spots) in Min6 cells in A-C. E-F. PLA immunofluorescence (red) and DAPI nuclear signals (blue) in Min6 cells treated with (E.) Palmitate control-BSA or (F.) palmitic acid (PA). G. Quantification of nuclei lacking at least 1–2 nuclear PLA signals (focal spots) in Min6 cells in E-F. n = 3; scale bar = 20 μm. ****, P < .0001 based on Student’s t-test.

    Article Snippet: After stimuli treatment for 4 hr in low (2.5 mM) or high (25 mM) glucose, the cells were formaldehyde fixed and then incubated with rabbit α-LDB1 (kind gift from Dr. Paul Love, NIH) and mouse α-ISL1 antibodies (39.4D5-c, Developmental Studies Hybridoma Bank) prior to PLA procedure.

    Techniques: Immunofluorescence, Control